英语翻译A cholesterol oxidase (COD) gene from Brevibacterium sp.(DQ345780) was expressed in Escherichia coli BL21 (DE3),an afﬁnity protocol was developed for the preparation,and industrial application of this method was of great potential.

来源：学生作业帮助网编辑：作业帮时间：2024/05/01 19:55:19

英语翻译A cholesterol oxidase (COD) gene from Brevibacterium sp.(DQ345780) was expressed in Escherichia coli BL21 (DE3),an afﬁnity protocol was developed for the preparation,and industrial application of this method was of great potential.
英语翻译
A cholesterol oxidase (COD) gene from Brevibacterium sp.(DQ345780) was expressed in Escherichia coli BL21 (DE3),an afﬁnity protocol was developed for the preparation,and industrial application of this method was of great potential.Riboﬂavin was chosen as the afﬁnity ligand,and it was coupled with Sepharose 4B through some spacers.With the afﬁnitymedium,the puriﬁcation process consisted of only one afﬁnity chromatography step to capture the target protein.The puriﬁed cholesterol oxidasewas 99.5% pure analyzed on HPLC Vydac C4 column,and 98% with SDS–PAGE analysis.The yield of the expressed enzyme was 9.8% of crude extracted proteins; the recovery of typical cholesterol oxidase activity was 90.1%,higher than that of other reported traditional protocols.Reducing SDS–PAGE analysis showed that the enzyme was a single polypeptide with the mass of ∼50 kDa.The desorption constant Kd and the theoretical maximum absorption Qmax on the afﬁnity medium were 1.0\2g/g medium and 74.5mg/g mediumin absorption analysis.Km and Vmax of cholesterol oxidase activity for the puriﬁed enzyme were 25.5\2M and 16.4\2mol/(minmg),respectively.
如果觉得麻烦可以只翻译前面三四句话就好了

英语翻译A cholesterol oxidase (COD) gene from Brevibacterium sp.(DQ345780) was expressed in Escherichia coli BL21 (DE3),an afﬁnity protocol was developed for the preparation,and industrial application of this method was of great potential.
短杆菌sp.(DQ345780)的胆固醇氧化酶基因在大肠杆菌BL21 (DE3)中进行表达,原位亲和就是为了准备这个实验而开发出来的.该方法的工业应用潜力巨大.选用核黄素作为亲和配体,通过垫片与琼脂糖4b结合.使用该亲和介质后,提纯过程只需亲和层析一个步骤就可完成目标蛋白的提取.提纯后的胆固醇氧化酶用高效液相色谱HPLC Vydac C4 column分析,纯度达到99.5%,用聚丙烯酰胺凝胶（SDS–PAGE ）分析,纯度达到98%.表达的酶量占粗提蛋白质的9.8%；典型胆固醇氧化酶活力恢复程度为90.1%,高于曾经报道过的传统原位技术的数据.简化SDS–PAGE分析表明该酶是一种质量为50kDa的单一多肽.吸收分析中,在亲和介质上解吸常数Kd和理论最大吸收量Qmax分别为1.0\2g/g介质和 74.5mg/g介质.经提纯的胆固醇氧化酶活性Km值和Vmax值分别为 25.5\2M和16.4\2mol/(minmg),

全部展开

胆固醇 oxidase（雪） Brevibacterium sp 的基因。（DQ345780) 在大肠杆菌 BL21(DE3),af 被表达？nity 记录为准备被发展，而且这一个方法的工业申请是有棒潜能的。Ribo？avin 当做 af 被选择？nity ligand，而且它经过一些取间隔的装置与 Sepharose 4 B 一起加倍。藉由 af？nitymedium，puri？阳离子程序有唯一的一 af？nity 套色版行走取得目标蛋白质。puri？ed 胆固醇 oxidasewas 是纯粹的 99.5% 以 SDS－页的分析在 HPLC Vydac C4 专栏，和 98% 上分析。被表达的酵素的生产量是 9.8% 的天然的吸取蛋白质；恢复典型的胆固醇 oxidase 活动是 90.1%, 比那高其他者报告传统的记录。减少 SDS－页的分析表示酵素是在块 polypeptide ～含 kDa 50 的。desorption 常数 Kd 和在 af 上的理论上的最大吸收 Qmax？nity 媒体是 1.0g│g 媒体和 74.5 毫克│g mediumin 吸收分析。Comoros , 科摩罗和胆固醇 oxidase 活动的 Vmax 为 puri？ed 酵素是 25.5M 和 16.4摩尔│( minmg）, 分别地。

收起

全部展开

短杆菌胆固醇氧化酶基因在大肠杆菌中表达，an afﬁnity protocol was developed for the preparation，这种方法在工业上的应用具有巨大潜力。核黄素是亲和配体，通过垫片配合琼脂糖4b，通过这个亲和介质，其纯化步骤只用一个亲和层析步骤就可捕获到目标蛋白。用高效液相色谱和聚丙烯酰胺凝胶分析，被纯化的胆固醇氧化酶的纯度分别达到99.5%和98%。表达的酶占原料提取蛋白的9.8%；其真正的胆固醇氧化酶活力恢复为90.1%，高于以报道过的传统纯化方法的酶活力。 Reducing SDS–PAGE 分析表明这种酶是一种50kDa的多肽。其在亲和层析上的解析常数和理论上的最大吸收尿流分别为1.0\2g/g medium 和 74.5mg/g。被纯化的胆固醇氧化酶的Km和Vmax值分别为 25.5\2M和16.4\2mol/(minmg),
其中肯定有翻译错误的地方啊

收起

cholesterol 英语翻译In adults ingesting a diet moderately rich in cholesterol,daily cholesterol production stands at about11–13 mg per kilogram of body weight.This meansthat our body does not require dietary cholesterolintake,since the liver produces chole 英语翻译keep their cholesterol under control or eat less fat. 英语翻译要用an ox 英语翻译Effects of Lactobacillus acidophilus 43121 and a Mixtureof Lactobacillus casei and Bifidobacterium longumon the Serum Cholesterol Level and Fecal Sterol Excretionin Hypercholesterolemia-Induced Pigs cholesterol是什么意思 cholesterol是什么意思 cholesterol screening cholesterol是什么意思 cholesterol是什么意思英语翻译Ox oi ngu chua noi chuyen yi e 1chuc.a n.t noi j e k hieu.e ngu roi ngu thay a n.t cho a ne.nho ox wa.hon a hon a ngu ngon. 英语翻译A cholesterol oxidase (COD) gene from Brevibacterium sp.(DQ345780) was expressed in Escherichia coli BL21 (DE3),an afﬁnity protocol was developed for the preparation,and industrial application of this method was of great potential. 英语翻译To facilitate standardization,in 1990 CDC organized the Cholesterol Reference Method Laboratory Network(CRMLN) to provide access to the NRS/CHOL for both manufacturers and clinical laboratories.The CRMLN has developed a method comparison cholesterol formula是什么意思英语翻译1 Saturated fats raise total blood cholesterol levels more than dietary cholesterol because they tend to boost both good high-density lipoproteins and bad low-density lipoproteins chlesterol.2 Trans-fatty acids are fats produced by heatin Summary:A method of extraction,purification and gas chromatographic analysis of five cholesterol o求翻译,准确翻译 only a handful of nuts is necessary to reduce cholesterol levels.High-density lipoproteins are labeled as good cholesterol because they remove excess cholesterol from your blood and protect you from having a heart attack or stroke.Nuts such as almo 英语翻译Over the last 20 years,statins,which include drugs such as Zocor,Pravachol and Lipitor,have had a remarkable impact on heart disease.These drugs rid the bloodstream of cholesterol,a fatty substance produced primarily by the liver and acqu